Skip to main content

SARS-CoV-2 infection and human semen: possible modes of contamination and transmission



Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), has turned into a global pandemic with multitudinous health impacts.

Main body

In light of the higher vulnerability of men to COVID-19 than women, there is rising concerns on the impact of SARS-CoV-2 infection on male fertility and possibilities of seminal contamination and transmission. The pandemic has attributed to the brief suspension of many fertility clinics and pathology laboratories, though many remained functional. Few reports reflect that SARS-CoV-2 can contaminate the semen of COVID-19 patients as well as that of recovering patients. The viral invasion into the testis may be due to the disrupted anatomical barriers of the testis by the inflammatory responses, and the persistence of the virus in the semen may be facilitated by the testicular immune privilege. Since SARS-CoV-2 is an enveloped RNA virus, it is also theoretically possible that this virus can remain viable in the semen samples even after cryopreservation with liquid nitrogen.


The present review emphasizes the possibilities of seminal dissemination of SARS-CoV-2 and thereby the chances of its sexual transmission. These perceptions and predictions are to facilitate immediate necessary actions to improvise the standard precautionary procedures for laboratory practices, including semen analysis or processing the semen sample for fertility treatments.


The pandemic of coronavirus disease 2019 (COVID-19) by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has taken the world to the edge of health emergency. This β-coronavirus was first identified in December 2019 in Wuhan city, China [1]. On 11th March 2020, COVID-19 was declared a pandemic by the World Health Organization [1]. SARS-CoV-2 belongs to the family of Coronaviridae, along with SARS-CoV and Middle East Respiratory Syndrome-Coronavirus (MERS-CoV). These viruses have been reported to cause similar morbific impacts on the lower respiratory system. Transmittable SARS-CoV had been detected in blood, urine, and stools while MERS-CoV had been found only in respiratory fluids [2]. Inexplicably, COVID-19 occurrence differs with gender, as men have been seen to be more susceptible to it than women [3]. In the light of higher vulnerability of men to COVID-19, there are rising concerns on the impact of SARS-CoV-2 infection on male fertility as well as possibilities of seminal contamination and transmission.

Fertility treatments have been put on ice by many countries to alleviate the risk of spreading the infection, following the guidelines by the prominent international professional bodies (e.g., the American Society of Reproductive Medicine and the European Society of Human Reproductive and Embryology). The virulence of SARS-CoV-2 along with the ignorance of its impacts on assisted reproduction, deferral was the safest course of action.

SARS-CoV-2 in testis and possibilities of seminal contamination

SARS-CoV-2 shares 79% similarity in genetic ancestry with the SARS-CoV, and both these viruses recognize the same cellular entry receptor, angiotensin converting enzyme receptor-2 (ACE2) [4, 5]. The transmembrane protease, serine-2 (TMPRSS2) mediates most of the priming of viral spike proteins with ACE2 [6]. The testes reportedly are among the tissues with the highest ACE2 mRNA and protein expressions, which brought along a possibility of potential threat to male fertility by SARS-CoV-2 infection [6]. Reports depict significantly higher expressions of ACE2 expression in the testes compared to that in the ovaries [7], which explain higher chances of the infection in the testes [8]. It is also evident that activation of TMPRSS2 gene is dependent upon androgen receptor. Moreover, for both androgen receptor and ACE2, gene loci are localized in chromosome-X. Genetic polymorphisms of this chromosome and subsequent endogenous androgen actions reportedly are associated with TMPRSS2 gene transcription, ACE2 activation, and thus facilitation of viral invasion in target cells [9]. SARS-CoV-2 invasion in testicular cells is not yet evidenced, with elusive concept on the testicular viral dynamics and an in situ hybridization reporting non-recognition of viral genomic materials by testicular cells [10]. However, immunohistochemistry showed IgG deposition in testicular cells [10], which is an evidence towards secondary immune reaction mediated testicular effects of SARS-CoV-2 [11]. The testicular effects of SARS-CoV-2 may be impaired Leydig cell, Sertoli cell, and sperm functions, possibly via oxidant-sensitive inflammatory pathways rather than via influence upon the endocrine axes [11,12,13] Although data supporting SARS-CoV-2 invasion in testicular cells are elusive, Zhao et al. had reported the presence of SARS-CoV in the Leydig cells and testicular epithelial cells [14].

The COVID-19 outbreak has been very sudden and novel in nature, and despite a great surge in research on SARS-CoV-2, information on most of its aspects remain elusive. Thus far, there are mixed findings regarding the presence of SARS-CoV-2 in semen. In 2005, the European Union issued guidelines for the mandatory screening of all the patients for three blood-borne viruses, namely the hepatitis B and C (HBV, HCV) and the human immunodeficiency virus (HIV), prior to embarking on any form of assisted reproductive techniques (ART). Recently, mosquito-borne viruses have also shown its effects on fertility [15]. For instance, an asymptomatic man for up to 1 year of his post recovery period has been detected with the Zika virus (ZIKV) RNA in the semen [16, 17]. Especially, viruses with high titers in blood (viremia) have a great probability of being shed into other body fluids such as sweat, urine, feces, breast milk, and semen. Around 27 viruses have been detected in human semen till date. Hence, there could be chances of SARS-COV-2 also shedding into semen and potentially remaining infective and precarious. Also, the immune responses in male reproductive tract, an inflammatory propagation that may alter the blood-testis barrier and the structural stability of the virus along with the viremia, contribute in the existence of viruses in semen [18]. However, it may not be requisite that the presence of viruses in semen is a function of cognate receptors for the infection within the testis or even the capability of the virus to replicate in the male reproductive system. Finally, considering the paucity of data available on SARS-CoV-2, it is circumspect to assimilate information from the viruses belonging to the same family.

The blood and feces samples in COVID-19 patients has shown the presence of the virus [5, 19]. It is notable that in fecal specimen very low titers of SARS-CoV-2 have been detected by RT-PCR with high cycle threshold/Cq values ranging from 36–38 [19]. Considering this, there is very minute risk of substantial shedding of this virus in the human semen. However, the spermatogonia, Leydig cells, and the Sertoli cells [6] have a dominating expression of ACE-2 bringing the reproductive organs into the target radius of SARS-CoV-2 infectivity. A study has reported the presence of viral RNA in the semen or the testicular tissue in one out of 10 deceased patients [20], and another including 34 patients with mild symptoms [21]. Though in these studies, small sample size and selection bias, including those with severe viremia, may have influenced the given results. But, another cohort study has reported the presence of SARS-CoV-2 in four out of 15 chronically ill and in two out of 23 recovering men [22], which reflect that SARS-CoV-2 can contaminate semen of COVID-19 patients as well as that of recovering patients (Table 1 [20,21,22,23,24,25,26,27,28,29,30,31,32];). The viral invasion into the testis may be due to the disrupted anatomical barriers of the testis by the inflammatory responses, and the persistence of the virus in the semen may be facilitated by the testicular immune privilege. However, several studies till date have denied the viral presence in the semen of men in different phases of recovery [20, 21, 23, 28,29,30, 32]. In brief, due to the lack of strong valid information from large-scale cohort studies, seminal presence of SARS-CoV-2 in the severely infected patients cannot be confirmed. However, the few abovementioned reports on seminal presence of the virus definitely raise concern regarding the sexual transmission of the virus. For further verification of this premise, detailed studies are required. As of now, there is no valid evidence of the virus surviving beyond several months after the infection, which was not the case with ZIKV [16, 17]. In a case study of six men who died of SARS-CoV infection, the number of spermatozoa severely was seen to decline along with the destruction of germ cells. Testicular autopsy showed orchitis in all the six patients, along with damaged blood-testis barrier and direct destruction of seminiferous epithelium [10]. There is also a possibility of hyperthermia due to fever, secondary infection, hypoxia, and steroids being the key mediators of testicular damage in SARS-CoV-2 patients [33]. Preliminary studies had also shown that COVID-19 could impact on the male reproductive system failure [34].

Table 1 Studies reporting the relation between COVID-19 and possible infectivity through semen and sexual transmission

Recently, a single-center observational study by Li et al. was conducted on recovering COVID-19 male patients as well as using testicular and epididymal tissues of deceased COVID-19 patients [27]. The study revealed testicular congestion, interstitial edema, exudation of red blood cells, and T-lymphocyte (CD3+) and macrophage infiltration in the testes of the deceased COVID-19 patients. These corroborations point towards increased inflammatory responses in the testis (orchitis) along with epididymides (epididymitis) [27]. The same study traced ACE2 protein expression in the testicular cells of men who succumbed to COVID-19 and reported high ACE2 expressions in the Leydig cells [27]. ACE2 mRNA expression has also been shown to be high in spermatogonia [6, 21]. All these evidences along with a prominent rise in apoptotic cells within seminiferous tubules in deceased COVID-19 patients owing to the substantial germ cell destruction suggest SARS-CoV-2-mediated impairment of spermatogenesis. For analysis of the effect of COVID-19 on testicular functions in the recovering patients, semen samples were collected from twenty-three COVID-19 inpatients [27]. A week prior to the semen collection, all the patients were tested positive for SARS-CoV-2 RNA in the throat swab. The semen samples tested negative for SARS-CoV-2 RNA, but semen analysis showed that nine out of 23 COVID-19 inpatients (39.1%) had sperm concentration below 15× 106/ml, which corresponded to the recommended standards of WHO for diagnosing oligozoospermia, and 60.9% had significantly higher number seminal leucocytes [27].

However, in situ hybridization gave no absolute evidence of the viral RNA in the testis. Xu and colleagues have reported that the elevated levels of lymphocytes and macrophages in the testicular intercellular tissue might be due to secondary inflammatory responses to SARS-CoV-2 [10]. When 34 males recovering from mild symptoms were examined, 20% of them reported scrotal discomfort implicative of orchitis but studies remained inconclusive [21]. In order to preserve fertility of the COVID-19-affected men, while taking into account their holistic well-being, health care professionals should probe and investigate for the mildest of symptoms and the slightest possibility of presence of similar disease sequelae.

Evidences pertaining to the immune-protection by the intact blood-testis barrier have ruled out the possibility of SARS-CoV-2 in semen of mildly affected men [21]. The actual risk lies with the patients who are severely affected due to exaggerated immune responses compromising the testicular immune privilege. However, studies on men at advanced stage of COVID-19 is not available owing to difficulties in obtaining semen samples from those men as it is very less likely for them to ejaculate. Furthermore, the ethical and practical challenges one may face while collection of the semen sample shackle the pursuit of further studies for verifiable evidence of the presence of this virus and its colligative consequences in the male reproductive tract.

SARS-CoV-2 transmission during semen analysis

Due to the prominence of the SARS-CoV-2 pandemic, many fertility clinics and pathology labs have undergone a brief suspension, though many other labs still remained functional. This resulted in the closure of many diagnostic semen analysis processes, yet many pathology labs continued to perform post-vasectomy semen analysis, as they are considered to cause minimum risk of transmission [35].

As discussed earlier, a couple of preliminary studies show the possibility of transmission of coronaviruses through semen [10, 22]. Thus, it is mandatory to take certain precautions during laboratory practices while performing any type of semen analysis or semen processing for fertility treatments. For this purpose, it is necessary to add new techniques to standard procedures. Perhaps it may be appropriate to apply triple gradient systems used in semen procedures, just like in removing HIV virus [36]. Taking into consideration the fact that SARS-CoV-2 is an airborne virus, henceforth, it is advisable for one to take proper care so as to avoid exposures to aerosols. While performing certain aerosol-generating events and using specimen containers used for the collection of semen and the tubes used for the sample centrifugation, one should take careful measures to avoid any sort of contamination of the sample from the external environment. It is also advised to keep fully compact and tightly closed lids of all the tubes and to use aerosol-tight caps of centrifuge bucket during centrifugation. Also, staffs working inside the laboratories of fertility clinics must wear appropriate personal protective equipment. Above discussed precautions must become the part of standard practice, if not already being into consideration.

SARS-CoV-2 and cryopreservation

While observing the forms of standard practices of cryopreserving viruses in the laboratories, it has been noticed that even at ultra-low temperatures most of the viruses remain viable if stored dried, or in appropriate protein concentrations, and also in pH ranging between 7 and 8 [37]. For example, the influenza virus (an enveloped RNA virus belonging to the orthomyxoviridae family) causes similar respiratory tract pathogenesis like SARS-CoV-2 and reportedly can survive even when stored in the vapor phase of liquid nitrogen for up to 40 years [38]. Although there is scanty empirical data, it is theoretically possible that this virus can also remain viable in semen sample even after cryopreservation with liquid nitrogen, as it is an enveloped RNA virus. Till date, there is no evidence in the field of ART regarding cross-contamination of one virus to the other in contrast with storing them in liquid nitrogen or vapor phase. However, ART cryobanks are ought to maintain careful measures while storing all the samples in hermetically sealed highly secured devices, with quarantined samples segregated in cryovessels with the help of complete screening procedures, while also stipulating regulations for the associated risks in transporting the cryopreserved samples [39].

Possibility of SARS-CoV-2 being sexually transmitted

Though SARS-CoV-2 has been detected in the semen of people who have or are recovering from the virus [40], there is currently no evidence that the SARS-CoV-2 virus is transmitted through semen or vaginal fluids. In a systematic review, Tur-Kaspa et al. have reported that SARS-CoV-2 infection is not a sexually transmitted disease (STD) [22, 41]. But, according to the information from MayoClinic, the Centers for Disease Control and Prevention has recommended to resume your normal activities (including sexual contact) following complete vaccination. They have also recommended to avoid sexual contact with anybody who does not live in the same family if the persons are not fully vaccinated [42]. However, as discussed earlier, there was evidence of SARS-CoV-2 infection with testis [40] that may attribute to disruption of blood-testis barrier leading to subsequent infection and inflammation [10, 43]. Given the presence of SARS-CoV-2 in several biological fluids, most prominently in the respiratory droplets, mucus, saliva, and feces, and the transmissibility of the virus within approximately 6 ft distance, physical sexual intimacy presents a high-risk scenario for viral transmission, particularly for non-monogamous partners who do not live with one another. Thus, further research is needed to determine if the COVID-19 virus could be transmitted sexually.


Most of the reports on SAR-CoV-2 infection in men recovering in different phases of COVID-19 have denied the presence of the virus in the seminal plasma. Moreover, the studies pertaining to SARS-CoV-2 and semen infectivity mostly have small volume of sample size and no long-term follow-up. Therefore, further studies are required to investigate the detailed information about SARS-CoV-2 infectivity, pathogenesis, and its transmission to comprehend the implication in both clinical and epidemiology. Since there is possibility of semen contamination and impaired fertility parameters via SARS-CoV-2 infection, it is still advised as a major precaution for men who are recovering from COVID-19, to undergo a semen analysis test to confirm whether their fertility status has been affected by the illness or not. Moreover, the pandemic-enforced suspension of fertility laboratories may impact certain cohorts of population suffering from subfertility who may develop irreversible infertility and are therefore in emergency need to preserve sperms or undergo assisted reproduction. Thus, out of all the uncertainties, this global pandemic turns out to be a testing time specifically for the fertility clinics to review their current practices in order to resume the fertility services and share their own perspectives with other professionals.

Availability of data and materials

Not applicable



Angiotensin converting enzyme 2


Assisted reproductive technology


Coronavirus disease 19


Hepatitis B virus


Hepatitis C virus


Middle East respiratory disease coronavirus


Ribonucleic acid


Reverse transcriptase polymerase chain reaction


Severe acute respiratory syndrome coronavirus 2


Transmembrane protease, serine 2


Zika virus


  1. World Health Organization (2020) Naming the coronavirus disease (COVID-19) and the virus that causes it [URL:] (Accessed August 24, 2020).

  2. Xu D, Zhang Z, Jin L, Chu F, Mao Y, Wang H, Liu M, Wang M, Zhang L, Gao GF, Wang FS (2005) Persistent shedding of viable SARS-CoV in urine and stool of SARS patients during the convalescent phase. Eur J Clin Microbiol Infect Dis 24(3):165–171.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. Jordan RE, Adab P, Cheng K (2020) Covid-19: risk factors for severe disease and death. Brit Med J.

  4. Jia HP, Look DC, Shi L, Hickey M, Pewe L, Netland J, Farzan M, Wohlford-Lenane C, Perlman S, McCray PB Jr (2005) ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epithelia. J Virol 79(23):14614–14621.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. Zhang W, Du RH, Li B, Zheng XS, Yang XL, Hu B et al (2020) Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg Microbes Infect 9(1):386–389.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. Wang Z, Xu X (2020) scRNA-seq profiling of human testes reveals the presence of the ACE2 receptor, a target for SARS-CoV-2 infection in spermatogonia, Leydig and Sertoli cells. Cells 9:920

    CAS  Article  Google Scholar 

  7. Shastri A, Wheat J, Agrawal S, Chaterjee N, Pradhan K, Goldfinger M et al (2020) Delayed clearance of SARS-CoV2 in male compared to female patients: high ACE2 expression in testes suggests possible existence of gender-specific viral reservoirs. medRxiv.

  8. Dutta S, Sengupta P (2021) SARS-CoV-2 and male infertility: possible multifaceted pathology. Reprod Sci. 28(1):23–26.

    CAS  Article  PubMed  Google Scholar 

  9. Wambier CG, Goren A (2020) SARS-COV-2 infection is likely to be androgen mediated. J Am Acad Dermatol. 83(1):308–309.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. Xu J, Qi L, Chi X, Yang J, Wei X, Gong E, Peh S, Gu J (2006) Orchitis: a complication of severe acute respiratory syndrome (SARS). Biol Reprod 74(2):410–416.

    CAS  Article  PubMed  Google Scholar 

  11. Sengupta P, Dutta S (2021) COVID-19 and hypogonadism: secondary immune responses rule-over endocrine mechanisms. Hum Fertil:1–6.

  12. Sengupta P, Dutta S (2020) Does SARS-CoV-2 infection cause sperm DNA fragmentation? Possible link with oxidative stress. Eur J Contracept Reprod Health Care 25(5):405–406.

    Article  PubMed  Google Scholar 

  13. Dutta S, Sengupta P (2020) SARS-CoV-2 infection, oxidative stress and male reproductive hormones: can testicular-adrenal crosstalk be ruled-out? J Basic Clin Physiol Pharmacol 31(6).

  14. Zhao J, Zhou G, Sun Y, Wang S, Yang J, Meng E, Pan D, Li WS, Zhou XS, Wang YD, Lu JY, Li N, Wang DW, Zhou BC, Zhang TH (2003) Clinical pathology and pathogenesis of severe acute respiratory syndrome. Chin J Exp Clin Virol. 17(3):217–221

    Google Scholar 

  15. Puggioni G, Pintus D, Melzi E, Meloni G, Rocchigiani AM, Maestrale C et al (2018) Testicular degeneration and infertility following arbovirus infection. J Virol. 92:e01131–e011348

    Article  Google Scholar 

  16. Kurscheidt FA, Mesquita CSS, Damke G, Damke E, Carvalho A, Suehiro TT et al (2019) Persistence and clinical relevance of Zika virus in the male genital tract. Nat Rev Urol 16(4):211–230.

    Article  PubMed  Google Scholar 

  17. Roychoudhury S, Das A, Sengupta P, Dutta S, Roychoudhury S, Choudhury AP, Ahmed AB, Bhattacharjee S, Slama P (2020) Viral pandemics of the last four decades: pathophysiology, health impacts and perspectives. Int J Env Res Pub Health 17(24):9411.

    CAS  Article  Google Scholar 

  18. Salam AP, Horby PW (2017) The breadth of viruses in human semen. Emerg Infect Dis 23(11):1922–1924.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Holshue ML, DeBolt C, Lindquist S, Lofy KH, Wiesman J, Bruce H, Spitters C, Ericson K, Wilkerson S, Tural A, Diaz G, Cohn A, Fox L, Patel A, Gerber SI, Kim L, Tong S, Lu X, Lindstrom S, Pallansch MA, Weldon WC, Biggs HM, Uyeki TM, Pillai SK, Washington State 2019-nCoV Case Investigation Team (2020) First case of 2019 novel coronavirus in the United States. New Eng J Med 382(10):929–936.

    CAS  Article  PubMed  Google Scholar 

  20. Song C, Wang Y, Li W, Hu B, Chen G, Xia P et al (2020) Detection of 2019 novel coronavirus in semen and testicular biopsy specimen of COVID-19 patients. medRxiv.

  21. Pan F, Xiao X, Guo J, Song Y, Li H, Patel DP, Spivak AM, Alukal JP, Zhang X, Xiong C, Li PS, Hotaling JM (2020) No evidence of SARS-CoV-2 in semen of males recovering from COVID-19. Fertil Steril 113(6):1135–1139.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. Li D, Jin M, Bao P, Zhao W, Zhang S (2019) Clinical characteristics and results of semen tests among men with coronavirus disease 2019. JAMA Network Open 3:e208292

    Article  Google Scholar 

  23. Nora H, Philippos E, Marcel A, Cornelius D, Dunja B-B, Ortwin A et al (2020) Assessment of SARS-CoV-2 in human semen-a cohort study. Fertil Steril 114(2):233–238.

    CAS  Article  Google Scholar 

  24. Segars J, Katler Q, McQueen DB, Kotlyar A, Glenn T, Knight Z, Feinberg EC, Taylor HS, Toner JP, Kawwass JF, American Society for Reproductive Medicine Coronavirus/COVID-19 Task Force (2020) Prior and novel coronaviruses, COVID-19, and human reproduction: what is known? Fertil Steril. 113(6):1140–1149.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. Paoli D, Pallotti F, Colangelo S, Basilico F, Mazzuti L, Turriziani O, Antonelli G, Lenzi A, Lombardo F (2020) Study of SARS-CoV-2 in semen and urine samples of a volunteer with positive naso-pharyngeal swab. J Endocrinol Invest 2020(12):1–4.

    CAS  Article  Google Scholar 

  26. Ma L, Xie W, Li D, Shi L, Mao Y, Xiong Y et al (2020) Effect of SARS-CoV-2 infection upon male gonadal function: a single center-based study. medRxiv.

  27. Li H, Xiao X, Zhang J, Zafar MI, Wu C, Long Y, Ly W, Pan F, Meng T, Zhao K, Zhou L, Shen S, Liu L, Liu Q, Xiong C (2020) Impaired spermatogenesis in COVID-19 patients.

  28. Kayaaslan B, Korukluoglu G, Hasanoglu I, Kalem AK, Eser F, Akinci E, Guner R (2020) Investigation of SARS-CoV-2 in semen of patients in the acute stage of COVID-19 infection. Urol Int. 104(9-10):678–683.

    CAS  Article  PubMed  Google Scholar 

  29. Guo L, Zhao S, Li W, Wang Y, Li L, Jiang S et al (2020) Absence of SARS-CoV-2 in semen of a COVID-19 patient cohort. Andrology. 9:42–47

    Article  Google Scholar 

  30. Temiz MZ, Dincer MM, Hacibey I, Azar RO, Celik C, Kucuk SH et al (2021) Investigation of SARS-CoV-2 in semen samples and the effects of COVID-19 on male sexual health by using semen analysis and serum male hormone profile: a cross-sectional, pilot study. Andrologia. 53:e13912

    CAS  Article  Google Scholar 

  31. Gacci M, Coppi M, Baldi E, Sebastianelli A, Zaccaro C, Morselli S et al (2021) Semen impairment and occurrence of SARS-CoV-2 virus in semen after recovery from COVID-19. Hum Reprod Published online February 1, 2021:deab026

  32. Ruan Y, Hu B, Liu Z, Liu K, Jiang H, Li H, Li R, Luan Y, Liu X, Yu G, Xu S, Yuan X, Wang S, Yang W, Ye Z, Liu J, Wang T (2021) No detection of SARS-CoV-2 from urine, expressed prostatic secretions, and semen in 74 recovered COVID-19 male patients: a perspective and urogenital evaluation. Andrology. 9(1):99–106.

    CAS  Article  PubMed  Google Scholar 

  33. Yang M, Chen S, Huang B, Zhong JM, Su H, Chen YJ, Cao Q, Ma L, He J, Li XF, Li X, Zhou JJ, Fan J, Luo DJ, Chang XN, Arkun K, Zhou M, Nie X (2020) Pathological findings in the testes of COVID-19 patients: clinical implications. Eur Urol Focus 6(5):1124–1129.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Youssef K, Abdelhak K (2020) Male genital damage in COVID-19 patients: are available data relevant? Asian J Urol.

  35. Royal College of Patholgy, Association of Clinical Biochemistry and Laboratory Medicine, Association of Clinical Pathologists (2020) Recommendations from RCPath and professional bodies [IBMS, ACP and ACB] [Available from:] Accessed 17 June 2021

  36. Zafer M, Horvath H, Mmeje O, van der Poel S, Semprini A, Rutherford G et al (2016) Effectiveness of semen washing to prevent HIV transmission and assist pregnancy in HIV-discordant couples: a systematic review and meta-analysis. Fertil Steril 105(3):645–655.e2.

    Article  PubMed  Google Scholar 

  37. Gould EA (1999) Methods for long-term virus preservation. Mol Biotechnol 13(1):57–66.

    CAS  Article  PubMed  Google Scholar 

  38. Merrill D, Wade C, Fahnestock P, Baker R (2012) Long-term and short-term stability of viruses depend on storage temperature and preservation methods. []. Accessed 17 June 2021

  39. Yakass MB, Woodward B (2020) COVID-19: should we continue to cryopreserve sperm during the pandemic? Reprod Biomed Online 40(6):905.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  40. Patel KP, Vunnam SR, Patel PA, Krill KL, Korbitz PM, Gallagher JP et al (2020) Transmission of SARS-CoV-2: an update of current literature. Eur J Clin Microbiol Infect Dis. 2020:1–7

    Google Scholar 

  41. Tur-Kaspa T, Hildebrand G, Cohen D, Tur-Kaspa I (2020) Is COVID-19 a sexually transmitted disease? A systematic review. Fertil Steril 114(3):e527–e528.

    CAS  Article  PubMed Central  Google Scholar 

  42. Marshall WF (2021) Sex and coronavirus: can you get COVID-19 from sexual activity? Can you get coronavirus from sex? Mayo Clinic, May 2021. [URL: ] (Last Accessed May 21, 2021)

  43. Rothe C, Schunk M, Sothmann P, Bretzel G, Froeschl G, Wallrauch C, Zimmer T, Thiel V, Janke C, Guggemos W, Seilmaier M, Drosten C, Vollmar P, Zwirglmaier K, Zange S, Wölfel R, Hoelscher M (2020) Transmission of 2019-nCoV infection from an asymptomatic contact in Germany. New Eng J Med 382(10):970–971.

    Article  PubMed  Google Scholar 

Download references


Not applicable



Author information

Authors and Affiliations



PS designed and planned the research. KB, PS, SD, and SMA wrote the article and made the final revisions. The last corrections and adjustments have been made by PS and SD. The authors read and approved the final manuscript.

Corresponding author

Correspondence to Lipika Das Mukhopadhyay.

Ethics declarations

Ethics approval and consent to participate

Not applicable

Consent for publication

Not applicable

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bhattacharya, K., Mukhopadhyay, L.D., Goswami, R. et al. SARS-CoV-2 infection and human semen: possible modes of contamination and transmission. Middle East Fertil Soc J 26, 18 (2021).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • COVID-19
  • Male infertility
  • SARS-CoV-2
  • Semen infection
  • Sexual transmission