Table 1 PCR primers for DAZ and DAZLA genes
Gene | Primer | Primer sequence (5′ to 3′) | Fragment length (bp) |
|---|---|---|---|
DAZ | sY254 | F: GGGTGTTACCAGAAGGCAAA R: GAACCGTATCTACCAAAGCAGC | 380bp |
sY255 | F: GTTACAGGATTCGGCGTGAT R: CTCGTCATGTGCAGCCAC | 123bp | |
DAZLA | A260G | F: CCT GTG TAT CTA ATT ATG ATG R: CCT TAA GTT TGT AAC AGG GCC | 264bp |
A386G | F: GAATGCTGAATTTTTACTCTTGAAG R: CTCTATACGTGGCTAGAGTTC | 181 bp | |
Exon 3 | F: TGGGGGAGAAATTGTCACAT R: CCCTTTGGACACACCAGTTC3 | 150bp |