Fig. 2From: Efficacy of density gradient centrifugation technique (DGC) in enhancing sperm cell DNA quality for assisted reproductive techniquea Percentage of abnormal chromatin structure as assessed by the SCD test and DNA denaturation as evaluated by the AO test in the three spermatic fractions (neat semen, 45% layer, and 90% layer). Sperm DNA denaturation as assessed by acridine orange in the initial semen sample (b) and after centrifugation in the 90% fraction (c). Sperm cell heads with double-stranded DNA were green (AO-green cell); those of single-stranded (denaturated) DNA were red (AO-red cell). Sperm chromatin structure assessed by the SCD technique test in the initial semen sample (d) and after centrifugation in the 90% fraction (e). Sperm cell heads with normal chromatin structure have large halos (Halo +), and those of abnormal chromatin structure have very little or no halos (Halo -)Back to article page