Influence of tobacco cigarette heavy smoking on DNA methylation patterns and transcription levels of MAPK8IP3, GAA, ANXA2, PRRC2A, and PDE11A genes in human spermatozoa

Laqqan, Mohammed M.; Yassin, Maged M.

doi:10.1186/s43043-021-00084-1

Middle East Fertility Society Journal

Table 6 Association between the methylation levels at different CpGs and basic semen parameters in the heavy smokers (n = 160)

From: Influence of tobacco cigarette heavy smoking on DNA methylation patterns and transcription levels of MAPK8IP3, GAA, ANXA2, PRRC2A, and PDE11A genes in human spermatozoa

DMC		Semen volume (ml)	Sperm concentration (Mill/ml)	Total sperm motility (%)	Sperm progressive motility (%)	Sperm non-progressive motility (%)	Non-motile sperm (%)	Sperm abnormal form (%)
Methylation level at CpGs in *MAPK8Ip3* gene-related amplicon
CpG1	r	−0.108	0.040	0.111	0.081	0.080	−0.104	0.184
CpG1	P-value	0.175	0.617	0.162	0.307	0.316	0.192	0.020
CpG2	r	−0.113	−0.131	0.032	0.109	−0.019	−0.054	0.094
CpG2	P-value	0.156	0.099	0.686	0.170	0.815	0.500	0.239
CpG3	r	−0.197	−0.216	0.059	0.186	−0.034	−0.075	0.014
CpG3	P-value	0.013	0.006	0.462	0.018	0.669	0.343	0.856
CpG4	r	−0.144	−0.254	0.023	0.147	−0.060	−0.017	−0.029
CpG4	P-value	0.069	0.001	0.769	0.064	0.449	0.832	0.713
CpG5	r	−0.011	−0.240	−0.041	0.095	−0.134	0.038	−0.143
CpG5	P-value	0.887	0.002	0.607	0.232	0.091	0.633	0.072
CpG6	r	0.042	−0.098	0.010	0.059	−0.020	−0.028	0.070
CpG6	P-value	0.600	0.216	0.897	0.460	0.800	0.728	0.378
CpG7	r	−0.046	−0.093	0.048	0.088	−0.006	−0.064	−0.042
CpG7	P-value	0.561	0.241	0.549	0.268	0.937	0.423	0.597
CpG8	r	−0.005	−0.050	0.020	0.016	0.004	−0.021	0.075
CpG8	P-value	0.949	0.530	0.803	0.837	0.958	0.790	0.343
CpG9	r	−0.097	−0.198	0.050	0.118	0.005	−0.065	0.062
CpG9	P-value	0.225	0.012	0.533	0.136	0.952	0.414	0.438
CpG10	r	−0.079	−0.095	0.035	0.129	−0.060	−0.035	0.103
CpG10	P-value	0.320	0.233	0.658	0.103	0.449	0.659	0.194
CpG11	r	−0.142	−0.220	0.032	0.155	−0.046	−0.054	0.050
CpG11	P-value	0.072	0.005	0.691	0.051	0.562	0.494	0.531
CpG12	r	−0.125	−0.167	0.000	0.116	−0.077	−0.011	0.039
CpG12	P-value	0.116	0.034	1.000	0.145	0.335	0.894	0.622
CpG13	r	−0.056	−0.222	−0.009	0.069	−0.056	−0.017	0.083
CpG13	P-value	0.480	0.005	0.912	0.389	0.484	0.829	0.299
CpG14	r	−0.115	−0.068	0.048	0.074	0.001	−0.032	−0.094
CpG14	P-value	0.146	0.395	0.549	0.354	0.992	0.686	0.235
CpG15	r	−0.095	−0.103	0.070	0.140	0.006	−0.078	0.144
CpG15	P-value	0.233	0.194	0.379	0.077	0.938	0.329	0.069
CpG16	r	−0.010	−0.219	−0.105	0.038	−0.158	0.082	−0.031
CpG16	P-value	0.904	0.005	0.185	0.632	0.046	0.300	0.696
CpG17	r	−0.043	−0.105	0.097	0.188	−0.022	−0.100	0.102
CpG17	P-value	0.593	0.186	0.222	0.017	0.784	0.207	0.199
CpG18	r	−0.148	−0.100	−0.022	0.015	−0.048	0.015	0.137
CpG18	P-value	0.062	0.209	0.778	0.851	0.546	0.852	0.085
CpG19	r	−0.016	−0.139	0.094	0.163	0.004	−0.087	0.172
CpG19	P-value	0.837	0.079	0.239	0.039	0.957	0.274	0.029
CpG20	r	−0.015	−0.134	−0.046	0.052	−0.082	0.052	0.132
CpG20	P-value	0.853	0.091	0.565	0.510	0.301	0.518	0.097
CpG21	r	−0.081	−0.245	0.026	0.080	−0.012	−0.048	0.157
CpG21	P-value	0.307	0.002	0.742	0.315	0.877	0.545	0.048
CpG22	r	−0.137	−0.219	−0.173	−0.008	−0.199	0.153	−0.047
CpG22	P-value	0.085	0.005	0.028	0.922	0.011	0.054	0.557
Methylation level at CpGs in *GAA* gene-related amplicon
CpG1	r	−0.010	−0.111	0.115	0.097	0.067	−0.098	0.075
CpG1	P-value	0.904	0.164	0.147	0.221	0.396	0.220	0.343
CpG2	r	−0.001	0.042	−0.042	−0.112	−0.028	0.068	−0.046
CpG2	P-value	0.985	0.598	0.601	0.160	0.728	0.392	0.563
CpG3	r	−0.009	−0.097	−0.163	−0.121	−0.189	0.181	0.005
CpG3	P-value	0.912	0.221	0.039	0.129	0.017	0.022	0.946
CpG4	r	−0.199	0.008	0.024	0.041	0.019	−0.032	0.086
CpG4	P-value	0.012	0.923	0.759	0.605	0.807	0.685	0.282
CpG5	r	0.021	0.121	0.039	−0.143	0.134	−0.018	−0.026
CpG5	P-value	0.788	0.127	0.622	0.070	0.092	0.826	0.744
CpG6	r	0.109	0.075	0.006	−0.093	0.098	−0.017	−0.067
CpG6	P-value	0.170	0.346	0.939	0.243	0.218	0.834	0.400
CpG7	r	0.167	0.119	0.020	−0.148	0.117	0.004	−0.054
CpG7	P-value	0.035	0.133	0.800	0.062	0.142	0.959	0.500
CpG8	r	0.065	0.169	0.005	−0.080	0.024	0.025	−0.131
CpG8	P-value	0.413	0.032	0.952	0.316	0.766	0.756	0.100
CpG9	r	−0.074	0.116	−0.083	−0.188	0.045	0.080	0.037
CpG9	P-value	0.349	0.143	0.299	0.017	0.574	0.316	0.641
CpG10	r	0.047	0.163	−0.041	−0.109	−0.009	0.067	−0.114
CpG10	P-value	0.557	0.040	0.610	0.169	0.914	0.398	0.149
Methylation level at CpGs in *ANXA2* gene-related amplicon
CpG1	r	0.051	0.062	0.114	0.089	0.159	−0.134	0.045
CpG1	P-value	0.519	0.435	0.152	0.265	0.044	0.092	0.575
CpG2	r	0.003	0.016	0.029	−0.081	0.104	−0.004	0.054
CpG2	P-value	0.966	0.843	0.712	0.311	0.192	0.963	0.496
CpG3	r	0.084	0.208	−0.112	−0.208	0.025	0.104	−0.019
CpG3	P-value	0.293	0.008	0.157	0.008	0.751	0.193	0.810
CpG4	r	−0.110	−0.058	−0.017	0.044	−0.043	−0.003	0.106
CpG4	P-value	0.167	0.466	0.831	0.585	0.586	0.970	0.183
Methylation level at CpGs in *PRRC2A* gene-related amplicon
CpG1	r	−0.043	0.122	−0.116	−0.178	−0.093	0.139	−0.093
CpG1	P-value	0.587	0.123	0.144	0.025	0.241	0.079	0.240
CpG2	r	0.058	−0.029	−0.032	−0.085	0.010	0.033	−0.096
CpG2	P-value	0.465	0.715	0.691	0.287	0.898	0.681	0.229
CpG3	r	0.047	−0.036	−0.069	−0.128	−0.025	0.084	−0.094
CpG3	P-value	0.552	0.647	0.383	0.106	0.751	0.293	0.235
Methylation level at CpGs in *PDE11A* gene-related amplicon
CpG1	r	−0.015	−0.143	−0.004	0.022	−0.012	0.005	−0.008
CpG1	P-value	0.846	0.072	0.958	0.778	0.878	0.950	0.922

Spearman’s test; r Correlation coefficient, DMC Differentially methylated CpGs; P ≥ 0.05, not significant; P < 0.05, significant

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