Influence of tobacco cigarette heavy smoking on DNA methylation patterns and transcription levels of MAPK8IP3, GAA, ANXA2, PRRC2A, and PDE11A genes in human spermatozoa

Middle East Fertility Society Journal

Table 4 Association between the semen parameters and other investigated parameters in the heavy smokers (n=160)

Variables	Male age (years)		Sperm chromatin non-condensation		Sperm DNA fragmentation		Global DNA methylation level
Variables	R	P-value	r	P-value	r	P-value	r	P-value
Semen volume (ml)	−0.114	0.151	−0.082	0.304	−0.060	0.453	0.082	0.300
Sperm concentration (Mill/ml)	−0.232	0.003	0.183	0.021	0.145	0.068	−0.066	0.405
Total sperm motility (%)	0.004	0.957	0.118	0.139	0.031	0.697	−0.098	0.216
Sperm progressive motility (%)	0.099	0.214	0.077	0.333	−0.071	0.376	−0.046	0.567
Sperm non-progressive motility (%)	−0.077	0.335	0.083	0299	0.078	0.329	−0.066	0.404
Non-motile sperm (%)	0.004	0.960	−0.094	0.236	−0.013	0.874	0.085	0.285
Sperm abnormal form (%)	0.024	0.767	0.121	0.126	−0.103	0.194	0.028	0.723

Spearman’s test; r, correlation coefficient; P ≥ 0.05, not significant; P < 0.05, significant