Table 1 Primer sequences, number of differentially methylated CpGs (DMCs), and PCR annealing temperatures used to amplify regions including the target CpGs analyzed by deep bisulfite sequencing
cgID | Chr. | nt (hg19) | AT (°C) | Product size (bp) | DMC | Primer sequence (5′–3′) | |
|---|---|---|---|---|---|---|---|
cg07869343 | chr16 | 1797050 | 52 | 334pb | 22 | F | GAGGTAAAGTGTAAAGTATT |
R | CTAAATAACCTATACTTCCA | ||||||
cg05813498 | chr17 | 78093353 | 55 | 268pb | 10 | F | AGTTGGGTATTAATTATTTTAA |
R | AACTCAAATACATACCCCAC | ||||||
cg09785377 | chr15 | 60644157 | 52 | 210pb | 4 | F | TGAGGAAAAATAATAAAGAGT |
R | CTAAACAATACCATTCAAAAC | ||||||
cg06833981 | chr6 | 31597708 | 54 | 206pb | 3 | F | TGATGATTTTTTTTTATGTGGATTT |
R | ACCCTTCCCTCATCACTATAA | ||||||
cg02745784 | chr2 | 178970365 | 52 | 398pb | 1 | F | AAAATGATGTAAAAATTAAAA |
R | TTTATATTCAAATCCATCCC | ||||||