Table 2 Study characteristics (excluded studies)
From: Correlation of sperm DNA damage with blastocyst formation: systematic review and meta-analysis
Serial no | Author and year | Type of assisted treatment | Type of study, no. of cycles | Sperm DNA fragmentation assay | Reason for exclusion |
|---|---|---|---|---|---|
1 | Benchaib et al. [3] | IVF/ICSI | Prospective study n = 104 cycles They have studied the formation of day 3 and not blastocysts | TUNEL | Insufficient data on blastulation rates and blastocyst formation rates cannot be calculated |
2 | Larson-Cook et al. [25] | IVF/ICSI | Retrospective n = 89cycles Blastulation rates with respect to high and low DFI are not mentioned | SCSA |  |
3 | Virro et al. [50] | IVF and or ICSi | Retrospective study n = 249 cycles Low DFI = 178 High DFI = 71 | SCSA |  |
4 | Huang et al. [22] | IVF/ICSI | Retrospective study n = 303cycles | TUNEL |  |
5 | Breznik et al. [37] | IVF/ICSI | Prospective study 883 cycles for IVF 878 cycles for ICSI | Sperm function tests | Â |
6 | Anifandis et al. [2] | IVF/ICSI | Prospective study n = 156 cycles Low DFI n = 71 High DFI n = 85 | SCD |  |
7 | Simon et al. [45] | IVF/ICSI | Prospective study n = 238 cycles only sperm DNA damage was studied using the 3 assays COMET assay n = 238 TUNEL assay n = 235 FCCE n = 102 | COMET TUNEL SCSA (FCCE) |  |
8 | Muriel et al. [30] | IVF/ICSI | Prospective study n = 85 cycles | SCD |  |
9 | Loutradi et al. [28] | ICSI | Prospective study n = 219 cycles Since semen analysis was used, we could not categorize as low DFI or high DFI | Semen analysis | Threshold for low and high DFI was not defined inappropriate inclusion of only high DFI Only patients with high DFI were considered and subjected to intervention |
10 | Greco et al. [19] | ICSI | prospective study n = 38 cycles | TUNEL | |
11 | Denomme et al. [11] | ICSI | Prospective study n = 246 cycles | Methylome and transcriptome analysis | DNA fragmentation index was not studied. Sperm DNA methylation defects were studied Since methylome and transcriptome analysis was done, there were no DFI categorization |
12 | Piccolomini et al. [36] | IVF/ICSI | Retrospective study n = 4205 cycles Since semen analysis was used, we could not categorize as low DFI or high DFI | Semen analysis as per WHO recommendations | DNA fragmentation index was not studied. Processed sperms were analyzed |
13 | Yang et al. [53] | ICSI | Prospective study n = 90 cycles High DFI = 60 Low DFI = 30 | DFI assay was not mentioned | Assay for sperm DNA fragmentation was not mentioned |