Table 1 The list of primers used for in tetra-primer ARMS-PCR method to detect the genotypes of GPX3 rs8177404 and rs3828599 polymorphisms
Polymorphism | System | Sequence | Allele |
|---|---|---|---|
rs8177404 | Forward inner primer | 5′GCGCATTTCCATGGTCTTCATAGAATC3′ | T |
Reverse inner primer | 5′TGGTCCTCTGGAAATTGAGGGTTTAAAA3′ | C | |
Forward outer primer | 5′ACCTTTTTCTACAGGCATGTCAGCTGTTAC3′ | Nonspecific allele | |
Reverse outer primer | 5′GTGCCTTTCCTCCCCATCATAATGA3′ | ||
rs3828599 | Forward inner primer | 5′CTAAATGGATGTGAAGCCACTTCGTCCA3′ | G |
Reverse inner primer | 5′CCAGCAAGTCCTGACTGGTTGTATGTGA3′ | A | |
Forward outer primer | 5′AGTCAGTCCCAACCTTCAGTTTTGGCAG3′ | Nonspecific allele | |
Reverse outer primer | 5′GCAGGGCCCAATTGTATCTTCTTTGAACT3′ |