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Middle East Fertility Society Journal
Fig. 2 | Middle East Fertility Society Journal

Fig. 2

From: Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Fig. 2

Histological picture to determine healthy primordial follicles. Formalin-fixed paraffin–embedded goat ovarian tissues were stained for HE (a, c, e, g) and TUNEL (b, d, f, h). Examination of HE observed both healthy primordial follicle cells (yellow arrows) and damaged primordial follicle cells with damage of membrane integrity or pyknotic cells (green arrows). In TUNEL staining, brown color primordial follicle cells show DNA fragmentation (white arrow). The proportion of primordial follicles in the fresh group was significantly higher compared to the vitrification group and vitrification and transplanted group (p < 0.05)

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